what is the benedict solution used to determine
Biochemical Test
Benedict's Examination Principle, Reagent Preparation, Process, Result, Limitation
Benedict'southward test is a chemic examination that is used to test for the presence of reduced sugars within an analytical examination. Thus,...
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Bridegroom'south Exam Principle, Reagent Preparation, Procedure, Effect, Limitation
Benedict's Test
Benedict'due south test is a chemical test that is used to examination for the presence of reduced sugars within an belittling exam. Thus, simple carbohydrates that contain an aldehyde or free ketone functional group are detected using this examination. The test is basing itself upon Benedict'south Reagent (also called Benedict's solution) which is a circuitous mix of sodium carbonate, sodium citrate, and the pentahydrate of copper(Two) Sulfate.
If exposed to sugars that are reduced, the reactions will be triggered by Benedict's reagent which causes a brick-red-colored precipitate that is an indication of Bridegroom'due south test is positive.
It is worth noting that Benedict's test may exist used to test for sugar levels in urine sample. Because this test can detect ketones, a-hydroxy ketones, and aldehydes. Since the glucose aldose, whose open-chain creates an aldehyde-aldehyde grouping information technology gives a positive result when glucose is detected inside the test. But, a positive reaction could as well be acquired due to the presence of homogentisic acrid, ascorbic acid, or other reducing substances in urine. Thus that a positive Benedict'southward test does not mean that the subject in question is diabetic.
Benedict'southward quantitative reagent has potassium thiocyanate. It's used to determine the amount of reduction sugar exists. This solution produces a copper thiocyanate crystal that is white and may exist utilized as a Titration. The titration procedure should be repeated using glucose solution 1% in identify of the exam sample to ensure calibration.
Objectives of Benedict's Examination
- To notice the amount or presence of reducing saccharide present in the mixture.
- To determine the concentration of glucose in the solution. It is quantifiable.
Principle of Benedict'southward Test
The solution is heated when simple carbohydrates and Benedict's solution are combined. transforms into brick red. This is due to the reducing properties in simple carbohydrates. It is believed that the copper (2) Ions in Bridegroom'south solution get reduced into Copper (I) Ions and cause the change in color.
Information technology is believed that the carmine copper(I) oxide that forms is non soluble in water, and it is precipitated out of the solution. This is why the precipitate is formed. The more the concentration of reduced sugar rises and the more close the final colour will be brick-cerise, and the more precipitate is formed. Sometimes, a brick-ruddy solid, copper oxide, crystallizes from the solution and is deposited in the bottom of the tube.
The element of group i properties of sodium carbonate provide the conditions that are needed to trigger the Redox reaction. The Sodium citrate complexes with copper (2) Ions so that they don't degrade to copper(I) Ions during storage.
Circuitous carbohydrates, such as starches volition not react positively to the Benedict's exam unless they're cleaved down via digestion or heating (try chewing on crackers before conducting this examination). Table Carbohydrate (disaccharide) is not-reducing sugar, and volition not react with Iodine or the Benedict Reagent. Sugar must exist broken down into its constituents fructose and glucose. The glucose test will be positive, merely the test for starch will be negative.
Benedict'southward Solution Composition
I liter of Benedict's Solution is made past combining 100 grams of anhydrous sodium carbonate, 173 grams of sodium citrate and 17.3 grams of copper(II) pentahydrate of sulfate.
Quality Checking: enedict'southward solution has a violet in hue. To determine the quality of Benedict's solution, put 5ml of Benedict's mixture in a test tube, and and so the temperature. If it does not change color, that means that information technology is pure.
Procedure of Benedict's Examination
Grooming of Benedict's Reagent
One liter of Benedict'southward Reagent can be fabricated using a mixture of 17.three grams of copper pentahydrate sulfate (CuSO4.5H2O), 100 grams sodium carbonate (Na2CO3) every bit well as 173 grams sodium citrate, in distillated water (required amount). This is because the copper(Two) sulfurate functions as a source for Cu2+ ions. The sodium carbonate acts as an alkaline medium besides every bit the sodium citrate form complexes when information technology comes in contact with the Cu2+ ions. Distilled water is utilized as solvent.
Pureness of Benedict's reagent may be verified through heating in the test tube. The absence of any change in the blue color of the solution after heating indicates that the reagent is of pure.
Testing for Reducing Sugars
- Approximately ane ml of sample is placed into a make clean exam tube.
- 2 milliliters (ten drops) of Bridegroom's Reagent (CuSO4) is put into the testing tube.
- Afterwards that, the solution will be heated by a h2o bath for about 3-5 minutes.
- Check for changes in color inside the the test tube or the germination of precipitate.
Observation and Results of Benedict'due south Test
- If the color after boiling turns green, then there will there be 0.i or 0.5 percent sugar in the solution.
- If the colour changes to yellow Then 0.5 to 1 percent saccharide levels are present.
- If it turns orange, it indicates that between 1 and one.5 percent sugar has been present.
- If the color changes from cerise to black, one.5 or ii.0 percent sugar levels are present.
- If the color changes from brick ruddy to blood-red, information technology signifies at that place is more carbohydrate than in the solution.
Positive Benedict's Exam: Develops reddish precipitate within three minutes. Reducing sugars present. Examples: Glucose
Negative Benedict's Examination: no change in color (Remains in bluish). Sugars that reduce sugars are non present. Example: Sucrose.
| Appearance of solution | The concentration of reducing carbohydrate (m%) | Interpretation |
| Brick cherry-red with heavy precipitate | 2% or >ii% | A big amount of reducing saccharide is nowadays |
| Brownish orangish with ruddy precipitate | 1.v% | A moderate corporeality of reducing sugar is present |
| Yellow with precipitate | one% | A minor amount of reducing sugar is present |
| Greenish blueish and cloudy | 0.5% | Traceable corporeality of reducing sugar is present |
| Dark-green blue with yellow precipitate | 0.25% | Traceable amount of reducing sugar is nowadays |
| Green with no precipitate | 0.one% | Traceable amount of reducing sugar is nowadays |
| Blue color or cloudy | Nil | No reducing sugar is present |
Limitation of Benedict'due south Test
The weaknesses of Benedict'south test tin be seen as follows:
- False-positive reactions during the test may be observed when certain drugs are such as streptomycin, isoniazid and salicylates penicillin, P-aminosalicylic acid are obtained.
- The chemical compounds present in concentrated urine can decrease Benedict's reaction, which comprises creatinine, urate, and ascorbic acrid (the reduction is only slight).
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